GFAP polyclonal antibody

Product Specifications

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

ALXDRD; GFAP

Swiss Prot

P14136

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human GFAP (NP_002046.1) .

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC

Dilution

WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Polyclonal Antibodies

Applications Notes

Western blot (WB) analysis of GFAP pAb at 1:1000 dilution Lane1:U-87MG whole cell lysate (30ug) Lane2:SHSY5Y whole cell lysate (30ug) Lane3:EC9706 whole cell lysate (30ug) Lane4:3T3-L1 whole cell lysate (30ug) Lane5:C6 whole cell lysate (30ug)