CRISPR-Cas9 SA Rabbit monoclonal antibody

Product Specifications

Background

The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the Streptococcus pyogenes CRISPR antiviral immunity system that provides adaptive immunity against extra chromosomal genetic material. CRISPR/Cas9 genome editing tools have been used in many organisms, including mouse and human cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9

Swiss Prot

J7RUA5

Reactivity

Staphylococcus aureus

Immunogen

A synthesized peptide derived from CRISPR-Cas9 SA

Conjugation

Unconjugated

Applications

WB IHC ICC/IF IP FC

Dilution

WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30 FC 1:50

Purity

Affinity-chromatography

Modification

Unmodified

Molecular Weight

124kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CRISPR-Cas9 SA Antibody detects endogenous levels of total CRISPR-Cas9 SA

Applications Notes

Western blot analysis of CRISPR-Cas9 SA expression in (1) 293T cell lysate transfected with CRISPR-Cas9 SA; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.