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Phospho-POLR2A (S2) Rabbit monoclonal antibody

Product Specifications

Background

During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

POLR2A; POLR2; RNA polymerase II CTD repeat YSPTSPS

Swiss Prot

P24928

Reactivity

Human Mouse Rat

Immunogen

A synthesized peptide derived from human POLR2A

Conjugation

Unconjugated

Applications

WB IHC ICC/IF IP FC

Dilution

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

Purity

Affinity-chromatography

Modification

Phosphorylated

Molecular Weight

270kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-POLR2A (S2) Antibody detects endogenous levels of total Phospho-POLR2A (S2)

Applications Notes

Western blot analysis of Phospho-POLR2A (S2) expression in (1) MCF7 cell lysate treated with Lambda Phosphatase; (2) MCF7 cell lysate.

Host or Source

Rabbit

Available Sizes

Curated Selection

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