Phospho-Raf1 (S43) Rabbit monoclonal antibody
Product Specifications
Background
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1
Swiss Prot
P04049
Reactivity
Human Mouse Rat
Immunogen
A synthesized peptide derived from human Phospho-Raf1 (S43)
Conjugation
Unconjugated
Applications
WB
Dilution
WB 1:500~1:1000
Purity
Affinity-chromatography
Modification
Phosphorylated
Molecular Weight
73kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-Raf1 (S43) Antibody detects endogenous levels of total Phospho-Raf1 (S43)
Applications Notes
Western blot analysis of Phospho-Raf1 (S43) expression in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate.
Host or Source
Rabbit
Available Sizes
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