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Cyclin D1 Rabbit monoclonal antibody

Product Specifications

Background

Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

CCND1; BCL1; D11S287E; PRAD1; U21B31; Cyclin D1

Swiss Prot

P24385

Reactivity

Human Mouse Rat

Immunogen

A synthesized peptide derived from human Cyclin D1

Conjugation

Unconjugated

Applications

WB IHC ICC/IF IP

Dilution

WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:100 IP 1:50

Purity

Affinity-chromatography

Modification

Unmodified

Molecular Weight

~ 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cyclin D1 Antibody detects endogenous levels of total Cyclin D1

Applications Notes

Western blot (WB) analysis of Cyclin D1 Rabbit monoclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (30ug) Lane2:A431 whole cell lysate (30ug) Lane3:The Liver tissue lysate of Mouse (30ug) Lane4:The Lung tissue lysate of Rat (30ug)

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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