Firefly Luciferase Rabbit monoclonal antibody

Product Specifications

Background

Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Luciferase; Firefly; Luciferin 4 monooxygenase

Swiss Prot

P08659

Reactivity

Firefly

Immunogen

A synthesized peptide derived from Firefly Luciferase

Conjugation

Unconjugated

Applications

WB ICC/IF

Dilution

WB 1:1000~1:5000 ICC/IF 1:50~1:200

Purity

Affinity-chromatography

Modification

Unmodified

Molecular Weight

55kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Firefly Luciferase Antibody detects endogenous levels of total Firefly Luciferase

Applications Notes

Western blot analysis of Firefly Luciferase Antibody expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.