Firefly Luciferase Rabbit monoclonal antibody
Product Specifications
Background
Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Luciferase; Firefly; Luciferin 4 monooxygenase
Swiss Prot
P08659
Reactivity
Firefly
Immunogen
A synthesized peptide derived from Firefly Luciferase
Conjugation
Unconjugated
Applications
WB ICC/IF
Dilution
WB 1:1000~1:5000 ICC/IF 1:50~1:200
Purity
Affinity-chromatography
Modification
Unmodified
Molecular Weight
55kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Firefly Luciferase Antibody detects endogenous levels of total Firefly Luciferase
Applications Notes
Western blot analysis of Firefly Luciferase Antibody expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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