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Phospho-PKR (T446) Rabbit monoclonal antibody

Product Specifications

Background

PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

E2AK2; E2AK2; EIF2AK1; EIF2AK2; MGC126524; PKR p68 kinase; PKR; PRKR

Swiss Prot

P19525

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-PKR (T446)

Conjugation

Unconjugated

Applications

WB IHC IP ChIP

Dilution

WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50

Purity

Affinity-chromatography

Modification

Phosphorylated

Molecular Weight

68kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-PKR (T446) Antibody detects endogenous levels of total Phospho-PKR (T446)

Applications Notes

Western blot analysis of PKR phosphorylation expression in HeLa cell lysate treated with Calyculin A and TNF-alpha.

Host or Source

Rabbit

Available Sizes

Curated Selection

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