CD47 Rabbit monoclonal antibody
Product Specifications
Background
CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin (SIRP) . CD47 is absent from Rh-null erythrocytes, but does play a role in cell adhesion in non-erythroid cells and may prevent premature elimination of erythrocytes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
CD47; IAP; Integrin Associated Protein; MER6 ; OA3; Protein MER6 ; Antigen identified by monoclonal antibody 1D8
Swiss Prot
Q08722
Reactivity
Human Mouse Rat
Immunogen
A synthesized peptide derived from human CD47
Conjugation
Unconjugated
Applications
WB ICC/IF FC
Dilution
WB 1:500~1:2000 ICC/IF 1:50~1:200 FC 1:50
Purity
Affinity-chromatography
Modification
Unmodified
Molecular Weight
~ 52 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CD47 Antibody detects endogenous levels of total CD47
Applications Notes
Western blot (WB) analysis of CD47 Rabbit monoclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate (30ug) Lane2:A549 whole cell lysate (30ug) Lane3:HCT116 whole cell lysate (30ug) Lane4:HEK293T whole cell lysate (30ug)
Host or Source
Rabbit
Available Sizes
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