CD86 Rabbit monoclonal antibody

Product Specifications

Background

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

CD86; B7-2; B70; CD28LG2; LAB72; MGC34413; CTLA4 counter receptor B72; CLS1; Ly58; MB7

Swiss Prot

P42081

Reactivity

Human Rat

Immunogen

A synthesized peptide derived from human CD86

Conjugation

Unconjugated

Applications

WB IHC ICC/IF IP FC

Dilution

WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

Purity

Affinity-chromatography

Modification

Unmodified

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD86 Antibody detects endogenous levels of total CD86

Applications Notes

Western blot (WB) analysis of CD86 Rabbit monoclonal antibody at 1:500 dilution Lane1:Raji whole cell lysate (30ug) Lane2:HuT78 whole cell lysate (30ug) Lane3:The Lung tissue lysate of Mouse (30ug) Lane4:CT-26 whole cell lysate (30ug)