Phospho-Histone H3 (S10) Rabbit monoclonal antibody
Product Specifications
Background
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
H3 histone, family 3A; H3 histone, family 3B (H3.3B) ; H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3
Swiss Prot
P68431
Reactivity
Human Mouse Rat
Immunogen
A synthesized peptide derived from human Phospho-Histone H3 (S10)
Conjugation
Unconjugated
Applications
WB IHC ICC/IF IP
Dilution
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
Purity
Affinity-chromatography
Modification
Phosphorylated
Molecular Weight
~ 18 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-Histone H3 (S10) Antibody detects endogenous levels of total Phospho-Histone H3 (S10)
Applications Notes
Western blot (WB) analysis of Histone H3 (Phospho-Ser10) Rabbit monoclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (30ug) Lane2:HEK293T treated with Calyculin A (50 nM,30 minutes) whole cell lysate (30ug) Lane3:Hela whole cell lysate (30ug) Lane4:The Lung tissue lysate of Mouse (30ug) Lane5:The Heart tissue lysate of Rat (30ug)
Host or Source
Rabbit
Available Sizes
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