CD31 polyclonal antibody

Product Specifications

Background

CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1

Swiss Prot

P16284

Reactivity

Human

Immunogen

Recombinant protein of human CD31.

Conjugation

Unconjugated

Applications

WB IP IF

Dilution

WB: 1:5000~1:10000 IP 1:50 - 1:200 IF 1:100 - 1:500

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 130 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD31 polyclonal antibody detects endogenous levels of CD31 protein.

Applications Notes

Western blot (WB) analysis of CD31 polyclonal antibody at 1:5000 dilution Lane1:Jurkat whole cell lysate (30ug) Lane2:THP-1 whole cell lysate (30ug) Lane3:A549 whole cell lysate (30ug)