CRHBP polyclonal antibody
Product Specifications
Background
Response to stress in mammals requires an intact hypothalamic-pituitary-adrenal axis. The proximal part of the response is mediated by secretion of corticotropin-releasing hormone (CRH) by the paraventricular nucleus of the hypothalamus. CRH is a 41 amino acid peptide derived by enzymatic cleavage from a 191 amino acid preprohormone. CRH is produced not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes; it is highly expressed in human placenta. Glucocorticoids stimulate placental CRH synthesis and secretion in primary cultures of human placenta. This stimulation is in contrast to the glucocorticoid suppression of CRH expression in hypothalamus. The gene which encodes CRH maps to human chromosome 8q13. Human plasma contains a CRH-binding protein, CRH-BP (also designated CRF-BP) which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. The gene which encodes CRF-BP maps to human chromosome 5q11.2-q13.3 .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
CRFBP; CRF-BP
Swiss Prot
P24387
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2) .
Conjugation
Unconjugated
Applications
WB IHC-P IF/ICC
Dilution
WB: 1:1000-1:5000 IHC-P: 1:100-1:500 IF/ICC: 1:50-1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 37 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CRHBP polyclonal antibody detects endogenous levels of CRHBP protein.
Applications Notes
Western blot analysis of Rat brain, using CRHBP pAb at 1:2000 dilution.
Host or Source
Rabbit
Available Sizes
Curated Selection
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