Tyk 2 polyclonal antibody

Product Specifications

Background

Tyrosine kinase of the non-receptor type involved in numerous cytokines and interferons signaling, which regulates cell growth, development, cell migration, innate and adaptive immunity.Plays both structural and catalytic roles in numerous interleukins and interferons (IFN-alpha/beta) signaling.Associates with heterodimeric cytokine receptor complexes and activates STAT family members including STAT1, STAT3, STAT4 or STAT6.The heterodimeric cytokine receptor complexes are composed of (1) a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1), and (2) a second receptor chain associated either with JAK1 or JAK2.In response to cytokine-binding to receptors, phosphorylates and activates receptors (IFNAR1, IL12RB1, IL10RB or IL13RA1), creating docking sites for STAT members.In turn, recruited STATs are phosphorylated by TYK2 (or JAK1/JAK2 on the second receptor chain), form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes.Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Non-receptor tyrosine-protein kinase TYK2

Swiss Prot

P29597

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to Human Tyk 2.

Conjugation

Unconjugated

Applications

WB IP

Dilution

WB: 1:1000~1:2000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 134 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Tyk 2 polyclonal antibody detects endogenous levels of Tyk 2 protein.

Applications Notes

Western blot (WB) analysis of Tyk 2 polyclonal antibody at 1:1000 dilution Lane1:SC7901 whole cell lysate (30ug) Lane2:SK-OVCAR3 whole cell lysate (30ug) Lane3:MCF-7 whole cell lysate (30ug)