NOX4 Rabbit monoclonal antibody

Product Specifications

Background

The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD (P) H-oxidase; NOX4; RENOX

Swiss Prot

Q9NPH5

Reactivity

Human

Immunogen

A synthesized peptide derived from human NOX4

Conjugation

Unconjugated

Applications

WB, IHC, ICC/IF, IP

Dilution

WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 62 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NOX4 Rabbit monoclonal antibody detects endogenous levels of NOX4 protein.

Applications Notes

Western blot (WB) analysis of NOX4 Rabbit monoclonal antibody at 1:5000 dilution. Lane1:SHSY5Y whole cell lysate (30ug) Lane2:HEK293T whole cell lysate (30ug) Lane3:MCF-7 whole cell lysate (30ug) Lane4:A549 whole cell lysate (30ug) Lane5:Hela whole cell lysate (30ug)