IκB-α (Phospho-S32/S36) polyclonal antibody
Product Specifications
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI
Swiss Prot
P25963
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human IκB-α around the phosphorylation site of Serine 32/Serine 36.
Conjugation
Unconjugated
Related Pathways
NF-kB Signaling, STING Signaling
Applications
WB
Dilution
WB 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 39 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IκB-α (Phospho-S32/S36) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at Ser32/Ser36.
Applications Notes
Western blot (WB) analysis of IκB-α (Phospho-S32/S36) polyclonal antibody at 1:200 dilution Lane1:THP-1 whole cell lysate (30ug) Lane2:THP-1 treated with PMA (200nM, 30min) whole cell lysate (30ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
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