Acetyl-Lysine Pan polyclonal antibody
Product Specifications
Background
Acetyllysine (or acetylated lysine) is an acetyl-derivative of the amino acid lysine. There are multiple forms of acetyllysine - this article refers to N-ε-acetyl-L-lysine. The other form is N-α-acetyl-L-lysine. In proteins, the acetylation of lysine residues is an important mechanism of epigenetics. It functions by regulating the binding of histones to DNA in nucleosomes and thereby controlling the expression of genes on that DNA. Non-histone proteins are acetylated as well. Unlike the functionally similar methyllysine, acetyllysine does not carry a positive charge on its side chain.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Reactivity
ALL
Immunogen
Purified protein corresponding to Acetyl Lysine.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB 1:1000 - 1:2000 IHC 1:200 - 1:500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Acetylated
Molecular Weight
Refer to Figures
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Acetyl-Lysine Pan polyclonal antibody detects endogenous levels of Acetyl-Lysine Pan protein.
Applications Notes
Western blot analysis of Acetyl Lysine expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.
Host or Source
Rabbit
Available Sizes
Curated Selection
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