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Acetyl-Lysine Pan polyclonal antibody

Product Specifications

Background

Acetyllysine (or acetylated lysine) is an acetyl-derivative of the amino acid lysine. There are multiple forms of acetyllysine - this article refers to N-ε-acetyl-L-lysine. The other form is N-α-acetyl-L-lysine. In proteins, the acetylation of lysine residues is an important mechanism of epigenetics. It functions by regulating the binding of histones to DNA in nucleosomes and thereby controlling the expression of genes on that DNA. Non-histone proteins are acetylated as well. Unlike the functionally similar methyllysine, acetyllysine does not carry a positive charge on its side chain.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Reactivity

ALL

Immunogen

Purified protein corresponding to Acetyl Lysine.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB 1:1000 - 1:2000 IHC 1:200 - 1:500

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Acetylated

Molecular Weight

Refer to Figures

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Acetyl-Lysine Pan polyclonal antibody detects endogenous levels of Acetyl-Lysine Pan protein.

Applications Notes

Western blot analysis of Acetyl Lysine expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Curated Selection

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