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PINK1 polyclonal antibody

Product Specifications

Background

PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria. PINK1 phosphorylates Parkin and promotes its translocation to mitochondria. Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein kinase PINK1 mitochondrial; BRPK; PTEN-induced putative kinase protein 1

Swiss Prot

Q9BXM7

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PINK1.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PINK1 polyclonal antibody detects endogenous levels of PINK1 protein.

Applications Notes

Western blot analysis of PINK1 expression in HEK293T (A), MCF7 (B), HCT116 (C), mouse heart (D), rat testis (E) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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