Ox-LDL monoclonal antibody

Product Specifications

Background

Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

Ox-LDL: LDL (Copper oxidized) ; Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein

Reactivity

Mouse, Rat

Immunogen

Full length protein from human plasma.

Conjugation

Unconjugated

Applications

WB IF/ICC IHC ELISA

Dilution

WB 1:500 - 1:2000 IHC 1:100 - 1:500 IF/ICC 1:100 - 1:200 ELISA 1:5000 - 1:10000

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 31 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ox-LDL monoclonal antibody detects endogenous levels of ox-LDL protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (Mouse liver) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ox-LDL) Monoclonal Antibody, Unconjugated at 1:2000 overnight at 4°C.