PCNA (I88) polyclonal antibody

Product Specifications

Background

The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Proliferating cell nuclear antigen; Cyclin

Swiss Prot

P12004

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 51-100 of Human PCNA.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas, DNA Methylation

Applications

WB IHC IP

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 34 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PCNA (I88) polyclonal antibody detects endogenous levels of PCNA protein.

Applications Notes

Western blot (WB) analysis of PCNA (I88) polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)