PPARγ (Phospho-Ser112) polyclonal antibody

Product Specifications

Background

Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Peroxisome proliferator-activated receptor gamma; Short name=PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3; PPAR γ; PPARγ

Swiss Prot

P37231

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic phosphopeptide derived from human PPARγ around the phosphorylation site of Serine 112.

Conjugation

Unconjugated

Related Pathways

MAPK Erk in Growth and Differentiation Pathway (, MTOR signaling

Applications

WB IP

Dilution

WB: 1:500~1:1000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 67 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PPARγ (Phospho-Ser112) polyclonal antibody detects endogenous levels of PPARγ protein only when phosphorylated at Ser112.

Applications Notes

Western blot (WB) analysis of PPARγ (Phospho-Ser112) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (30ug) Lane2:MCF-7 treated with λ-phosphatase whole cell lysate (30ug) Lane3:PC3 whole cell lysate (30ug) Lane4:PC3 treated with λ-phosphatase whole cell lysate (30ug) Lane5:CT-26 whole cell lysate (30ug) Lane6:PMVEC whole cell lysate (30ug)