NEK2 polyclonal antibody
Product Specifications
Background
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
NLK1; RP67; NEK2A; HsPK21; PPP1R111; NEK2
Swiss Prot
P51955
Reactivity
Human, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 345-445 of human NEK2 (NP_002488.1) .
Conjugation
Unconjugated
Applications
WB, IHC-P
Dilution
WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 52 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NEK2 polyclonal antibody detects endogenous levels of NEK2 protein.
Applications Notes
Western blot analysis of lysates from HeLa cells, using NEK2 pAb at 1:1000 dilution.
Host or Source
Rabbit
Available Sizes
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