CLIC1 polyclonal antibody

Product Specifications

Background

Chloride intracellular channel 1 (CLIC1), also referred to as NCC27, is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. CLIC1 is a monomeric protein that contains a redox-active site similar to glutaredoxin; it functions as a anionselective channel. CLIC1 forms a dimer when oxidized and is then able to form chloride ion channels in bilayers and vesicles, whereas a reducing environment prevents this from occurring. Insulin concentration also plays a role in CLIC1 regulation, and the hormone may cause a subnuclear relocalization of CLIC1. CLIC1 is associated with macrophage activation; a downregulation of CLIC1 function prevents TNFα release induced by β-Amyloid protein (A-β) stimulation. This suggests a role for CLIC1 in several neurodegenerative processes, such as Alzheimer’s disease, a syndrome characterized by an accumulation of A-β.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Chloride intracellular channel protein 1; Chloride channel ABP; Nuclear chloride ion channel 27; NCC27; Regulatory nuclear chloride ion channel protein; hRNCC; CLIC1; G6; NCC27

Swiss Prot

O00299

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Protein corresponding to a sequence within amino acids 42-241 of human CLIC1 (NP_001279.2) .

Conjugation

Unconjugated

Applications

WB IF/ICC IP

Dilution

WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 IP 1:50 - 1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .