SUMO-2 (G93) polyclonal antibody
Product Specifications
Background
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer’s disease.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Small ubiquitin-related modifier 2; SUMO-2; HSMT3; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A; Smt3A; SUMO2; SMT3A; SMT3H2; SUMO3
Swiss Prot
P61956
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 60-110 of Human SUMO-2.
Conjugation
Unconjugated
Applications
WB IHC IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 17 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SUMO-2 (G93) polyclonal antibody detects endogenous levels of SUMO-2 protein.
Applications Notes
Western blot (WB) analysis of SUMO-2 (G93) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:CT-26 whole cell lysate (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
