F12 (HC) polyclonal antibody

Product Specifications

Background

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants which are involved in a blood coagulation cascade leading to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Factor XII, (FXII) a blood coagulation factor, is a serum glycoprotein that participates in fibrinolysis, as well as the generation of bradykinin and angiotensin. An enzyme of the serine protease (or serine endopeptidase) class, it activates both Factor XI and prekallikrein in the coagulation cascade. Factor XII deficiency, a rare hereditary disorder slightly more prevalent among Asians, does not cause excessive hemorrhaging since other coagulation factors compensate for it. Researchers have still reported Factor XII deficiency to be a risk factor for the development of arterial and venous thromboembolism. The gene for human Factor XII maps to the very end of the long arm of the fifth chromosome (5q33-qter) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Coagulation factor XII; Hageman factor; HAF; Coagulation factor XIIa heavy chain

Swiss Prot

P00748

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human F12 (HC) .

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 68 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

F12 (HC) polyclonal antibody detects endogenous levels of Coagulation factor XIIa heavy chain protein.

Applications Notes

Western blot (WB) analysis of F12 (HC) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)