Cathepsin D (HC) polyclonal antibody

Product Specifications

Background

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. Cathepsins may also cleave some protein precursors, thereby releasing regulatory peptides. The promoter region of the cathepsin D gene contains five Sp1 binding sites and four AP-2 binding sites.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cathepsin D; Cathepsin D heavy chain; CTSD; CPSD

Swiss Prot

P07339

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human Cathepsin D (Heavy chain) .

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 28, 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cathepsin D (HC) polyclonal antibody detects endogenous levels of Cathepsin D heavy chain (27 kDa) Protein.

Applications Notes

Western blot (WB) analysis of Cathepsin D (HC) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:The Embryo tissue lysate of Mouse (20ug) Lane4:The Lung tissue lysate of Rat (40ug)