MRPL54 polyclonal antibody
Product Specifications
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
39S ribosomal protein L54, mitochondrial; L54mt; MRP-L54
Swiss Prot
Q6P161
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to amino acids 83-127 of Human MRPL54.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 15 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MRPL54 polyclonal antibody detects endogenous levels of MRPL54 protein.
Applications Notes
Western blot (WB) analysis of MRPL54 pAb at 1:500 dilution Lane1:H1792 whole cell lysate (40ug) Lane2:Jurkat whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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