MIA polyclonal antibody

Product Specifications

Background

Tumorigenesis is a process that is mediated by a network of membrane, cytosolic and nuclear associated factors, which regulate proliferation and cell-matrix interaction through signaling cascades. The phenotype of malignant melanomas in vivo depends on the global expression of stimulatory or inhibitory factors generated in both the tumors cells and their environment. One example, Melanoma inhibitory activity (cartilage-derived retinoic acid-sensitive protein (CD-RAP), MIA) is a Src homology 3 (SH3) -like domain containing protein that is secreted from chondrocytes and malignant melanoma cells. MIA is translated as a 131-amino acid pro-form and processed into a mature 107-amino acid protein after cleavage of a secretion signal. MIA is expressed during chondrogenesis and in mature chondrocytes, suggesting that MIA is necessary for normal cartilage cell phenotype. MIA mRNA is present in carcinomas of the colon, ovary, kidney, and head/neck, and may represent a marker to monitor melanomic activity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Melanoma-derived growth regulatory protein; Melanoma inhibitory activity protein; MIA

Swiss Prot

Q16674

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human MIA.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 17 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MIA polyclonal antibody detects endogenous levels of MIA protein.

Applications Notes

Western blot (WB) analysis of MIA pAb at 1:500 dilution Lane1:BV2 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:A2780 whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug) Lane6:HEK293T whole cell lysate (40ug)