ZC3H8 polyclonal antibody

Product Specifications

Background

Zinc-finger CCCH domain-containing protein 8 (ZC3H8) acts as a transcriptional repressor of the GATA3 promoter. It is a sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (by similarity) . ZC3H8 induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Zinc finger CCCH domain-containing protein 8; ZC3HDC8

Swiss Prot

Q8N5P1

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 133-178 of Human ZC3H8.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ZC3H8 polyclonal antibody detects endogenous levels of ZC3H8 protein.

Applications Notes

Western blot (WB) analysis of ZC3H8 pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (20ug) Lane2:Hela whole cell lysate (20ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)