CES2 polyclonal antibody

Product Specifications

Background

CES1 and CES2 are the two major liver carboxylesterases which belong to the type-B carboxylesterase/lipase family. Helping the body in the detoxification of a wide range of xenobiotics, CES1 and CES2 are involved in the hydrolyzing activation of therapeutic ester and amide pro-drugs, as well as in the detoxification of several narcotic compounds. The catalytic activity of CES1 and CES2 is influenced by both the esterification site and the structure/moiety of the amino acid. While CES1 shows high affinity for aromatic and aliphatic esters, CES2 shows high affinity for 3,6-diacetyl and 6-monoacetly esters, such as those found in morphine and morphine derivatives.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cocaine esterase; Carboxylesterase 2; CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2; CES-2; ICE

Swiss Prot

O00748

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to amino acids 379-424 of Human CES2.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CES2 polyclonal antibody detects endogenous levels of CES2 protein.

Applications Notes

Western blot (WB) analysis of CES2 (Q369) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug)