FoxO3A (phospho-S253) polyclonal antibody

Product Specifications

Background

FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1; FOXO3A

Swiss Prot

O43524

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human FOXO3A around the phosphorylation site of Serine 253.

Conjugation

Unconjugated

Related Pathways

Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 80-95 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-FoxO3A (S253) polyclonal antibody detects endogenous levels of FoxO3A protein when phosphorylated at Ser253.

Applications Notes

Western blot (WB) analysis of p-FoxO3A (S253) pAb at 1:500 dilution Lane1:The Testis tissue lysate of Rat (40ug) Lane2:The Testis tissue lysate of Mouse (20ug) Lane3:Hela whole cell lysate (40ug)