Rrn3 (phospho-S649) polyclonal antibody
Product Specifications
Background
Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC) . Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA; RRN3; TIFIA
Swiss Prot
Q9NYV6
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human Rrn3 around the phosphorylation site of Serine 649
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 74 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Rrn3 (phospho-S649) polyclonal antibody detects endogenous levels of Rrn3 protein only when phosphorylated at Ser649.
Applications Notes
Western blot (WB) analysis of Rrn3 (phospho-S649) polyclonal antibody at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug) Lane6:HCT116 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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