Ku-70 (phospho-S5) polyclonal antibody
Product Specifications
Background
The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 (or p86), which is also known by the synonym Ku-80 (or p80) . Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in; Chinese hamster cells 6; XRCC6; G22P1
Swiss Prot
P12956
Reactivity
Human
Immunogen
Synthetic peptide derived from human Ku-70 around the phosphorylation site of S5.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 70 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Ku-70 (phospho-S5) polyclonal antibody detects endogenous levels of Ku-70 protein around the phosphorylation site of S5.
Applications Notes
Western blot (WB) analysis of p-Ku-70 (S5) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:A375 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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