ERβ (phospho-S105) polyclonal antibody

Product Specifications

Background

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2; ESR2; ESTRB; NR3A2; ER-β

Swiss Prot

Q92731

Reactivity

Human

Immunogen

Synthetic peptide derived from human ERβaround the phosphorylation site of S105.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ERβ (phospho-S105) polyclonal antibody detects endogenous levels of Erβprotein around the phosphorylation site of S105.

Applications Notes

Western blot (WB) analysis of p-ERβ (S105) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (40µg) Lane2:SK-OVCAR3 whole cell lysate (40µg) Lane3:MCF-7 whole cell lysate (40µg) Lane4:PC3 whole cell lysate (40µg) Lane5:LO2 whole cell lysate (40µg)