TH (phospho-S8) polyclonal antibody

Product Specifications

Background

The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH; TYH

Swiss Prot

P07101

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human TH around the phosphorylation site of S8.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas, Dopamine Signaling in Parkinson's Disease Plathway

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 65 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TH (phospho-S8) polyclonal antibody detects endogenous levels of TH protein only when phosphorylated at Ser8.

Applications Notes

Western blot (WB) analysis of p-TH (S8) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:MCF-7 treated with Forskolin (4µg/ml,30 minutes) whole cell lysate (40ug) Lane3:MCF-7 treated with Forskolin (4µg/ml,60 minutes) whole cell lysate (40ug)