Eg5 (phospho-T926) polyclonal antibody
Product Specifications
Background
Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules. Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; KIF11; EG5; KNSL1; TRIP5
Swiss Prot
P52732
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Threonine 926
Conjugation
Unconjugated
Related Pathways
Regulation of Microtubule Dynamics
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 120 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Eg5 (T926) pAb detects endogenous levels of Eg5 protein only when phosphorylated at Thr926.
Applications Notes
Western blot (WB) analysis of p-Eg5 (T926) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40µg) Lane2:HepG2 whole cell lysate (40µg) Lane3:CT-26 whole cell lysate (40µg) Lane4:C6 whole cell lysate (40µg) Lane5:LOVO whole cell lysate (40µg) Lane6:MCF-7 whole cell lysate (40µg)
Host or Source
Rabbit
Available Sizes
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