APC1 (phospho-S688) polyclonal antibody
Product Specifications
Background
APC1 is 1 of at least 11 subunits of the anaphase promoting complex (APC), which functions at the metaphase to anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Anaphase-promoting complex subunit 1; APC1; Cyclosome subunit 1; Mitotic checkpoint regulator; Testis-specific gene 24 protein; ANAPC1; TSG24
Swiss Prot
Q9H1A4
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human APC1 around the phosphorylation site of Serine 688
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 216 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-APC1 (S688) pAb detects endogenous levels of APC1 protein only when phosphorylated at Ser688.
Applications Notes
Western blot (WB) analysis of p-APC1 (S688) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40µg) Lane2:K562 whole cell lysate (40µg) Lane3:HEK293T whole cell lysate (40µg)
Host or Source
Rabbit
Available Sizes
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