Tuberin (phospho-T1462) polyclonal antibody

Product Specifications

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors. Tuberin is directly phosphorylated at Thr1462 by Akt/PKB. Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity. In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tuberin; Tuberous sclerosis 2 protein; TSC2; TSC4

Swiss Prot

P49815

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human Tuberin around the phosphorylation site of Threonine 1462.

Conjugation

Unconjugated

Related Pathways

Insulin &Glucose Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 220 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Tuberin (T1462) pAb detects endogenous levels of Tuberin protein only when phosphorylated at Thr1462.

Applications Notes

Western blot (WB) analysis of p-Tuberin (T1462) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug)