LAT (phospho-Y191) polyclonal antibody
Product Specifications
Background
LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38; LAT
Swiss Prot
O43561
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human LAT (Isoform Short) around the phosphorylation site of Tyrosine 191.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 38 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-LAT (Y191) polyclonal antibody detects endogenous levels of human LAT (Isoform Short) only when phosphorylated at Tyr191, and detects LAT (Isoform Long) only when phosphorylated at Tyr220.
Applications Notes
Western blot (WB) analysis of p-LAT (Y191) pAb at 1:1000 dilution Lane1:H9C2 whole cell lysate (40ug) Lane2:RAW264.7 whole cell lysate (40ug) Lane3:K562 whole cell lysate (20ug) Lane4:Myla2059 whole cell lysate (20ug) Lane5:HuT78 whole cell lysate (20ug)
Host or Source
Rabbit
Available Sizes
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