Casein Kinase IIβ (phospho-S209) polyclonal antibody

Product Specifications

Background

CK2 (formerly called Casein Kinase II) is a highly conserved protein kinase with more than 300 substrates regulating cell growth, cell death, and cell survival. CK2 has been implicated in the response to UV irradiation-induced DNA damage, targeting XRCC1 and BRCA1 as well as regulating p53 tumor suppressor protein functions.Furthermore, CK2 plays a key role in NF-κB activation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8; KRT8; CYK8

Swiss Prot

P67870

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of Serine 432

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 27 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -21°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Casein Kinase IIβ (phospho-S209) polyclonal antibody detects endogenous levels of Casein Kinase IIβ protein only when phosphorylated at Ser209.

Applications Notes

Western blot (WB) analysis of Casein Kinase IIβ (phospho-S209) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate (40µg) Lane2:Panc1 whole cell lysate (40µg) Lane3:U-87MG whole cell lysate (40µg) Lane4:The Brain tissue lysate of Rat (40µg) Lane5:The Kidney tissue lysate of Mouse (40µg)