Troponin I3 (phospho-T142) polyclonal antibody
Product Specifications
Background
Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC) . Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Troponin I, cardiac muscle; Cardiac troponin I; TNNI3; TNNC1
Swiss Prot
P19429
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Troponin I3 around the phosphorylation site of Thr142.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Troponin I3 (phospho-T142) polyclonal antibody detects endogenous levels of Troponin I3 protein only when phosphorylated at Thr142.
Applications Notes
Western blot (WB) analysis of p-Troponin I3 (T142) pAb at 1:500 dilution Lane1:The Heart tissue lysate of Mouse (40ug) Lane2:The Heart tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
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