P70 S6K (phospho-S418) polyclonal antibody

P70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5' oligopyrimidine tract mRNAs. A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal.Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region. Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression. Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase.

Ribosomal protein S6 kinase I; S6K; S6K1; 70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase alpha; p70 S6K; p70-S6K; P70S6K; p70 S6 kinase α; Name: RPS6KB1; STK14A; p80 S6K1; p85 S6 kinase; p85 S6K; p85S6K

PI3K AKT signaling, Protein Kinase C Signaling, Hypoxia Signaling, Translational Contral elF4 and p70 S6 Kinase, Inhibition of Apoptosis, Insulin &Glucose Signaling, MAPK Erk in Growth and Differentiation Pathway, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

P-p70 S6K (S418) polyclonal antibody detects endogenous levels of p70 S6 kinase only when phosphorylated at Ser418. This antibody also detects p85 S6 kinase when phosphorylated at Ser441.

Western blot (WB) analysis of p-p70 S6 kinase α (S418) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)