IRS-1 (phospho-S323) polyclonal antibody

Product Specifications

Background

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4) . IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Insulin receptor substrate 1; IRS-1; IRS1

Swiss Prot

P35568

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 323.

Conjugation

Unconjugated

Related Pathways

Translational Contral elF4 and p70 S6 Kinase, Insulin &Glucose Signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 180 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-IRS-1 (S323) polyclonal antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser323.

Applications Notes

Western blot (WB) analysis of p-IRS-1 (S323) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:L02 treated with PBS (1xPBS, PH7.4) for 1 houre whole cell lysate Lane3:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 5min whole cell lysate Lane4:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 10min whole cell lysate Lane5:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 15min whole cell lysate