Dynamin-1 (phospho-S774) polyclonal antibody

Product Specifications

Background

Members of the Dynamin family, including Dynamin I and Dynamin II, are GTPase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin I is localized to the central nervous system, while Dynamin II exhibits ubiquitous distribution with highest expression in testis. Both Dynamin proteins contain SH3 and proline-rich domains that mediate interactions between the Dynamins and effectors of their GTPase activity. The interactions with these effectors, which include microtubules, acidic phospholipids and SH3 domain-containing proteins, are required for rapid endocytosis. Dynamin I appears to be recruited to Clathrin coated pits by SH3 domain interaction with Amphiphysin, a protein highly expressed in brain.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Dynamin 1; Dynamin1; DNM1; DNM; Dynamin I; Dynamin-I; DynaminI

Swiss Prot

Q05193

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Dynamin-1 around the phosphorylation site of Serine 774.

Conjugation

Unconjugated

Related Pathways

Vesicle Trafficking presynaptic signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 98 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Dynamin-1 (S774) polyclonal antibody detects endogenous levels of Dynamin-1 protein when phosphorylated at Ser774.

Applications Notes

Western blot (WB) analysis of p-Dynamin I (S774) pAb at 1:1000 dilution Lane1:The Brain tissue lysate of Mouse (30ug) Lane2:The Brain tissue lysate of Rat (30ug) Lane3:U-87MG whole cell lysate (40ug)