Daxx (phospho-S668) polyclonal antibody

Product Specifications

Background

Apoptosis, or programmed cell death, occurs duringnormal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED3 protease family. A novel DD containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx is a death domain containing important intermediate in the Fas mediated apoptosis. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N terminal kinase (JNK) pathway. It is widely expressed in fetal and adult human and mouse tissue, indicating its important function in Fas signaling pathways.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Death domain-associated protein 6; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein; BING2; DAP6; DAXX

Swiss Prot

Q9UER7

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human Daxx around the phosphorylation site of Serine 668.

Conjugation

Unconjugated

Related Pathways

Death Receptor Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 110 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Daxx (phospho-S668) polyclonal antibodydetects endogenous levels of Daxx protein only when phosphorylated at Ser668.

Applications Notes

Western blot (WB) analysis of p-Daxx (S668) pAb at 1:500 dilution Lane1:A2780 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:K562 whole cell lysate (20ug)