C/EBP α (phospho-S21) polyclonal antibody

C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of C/EBP α (phospho-S21) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 5 minutes whole cell lysate Lane3:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 10 minutes whole cell lysate Lane4:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 15 minutes whole cell lysate