C/EBP α (phospho-S21) polyclonal antibody

Product Specifications

Background

C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; C/EBP α; C/EBPα

Swiss Prot

P49715

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human C/EBP α around the phosphorylation site of Serine 21.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 40 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-C/EBP α (S21) polyclonal antibody detects endogenous levels of C/EBP α protein only when phosphorylated at Ser21.

Applications Notes

Western blot (WB) analysis of C/EBP α (phospho-S21) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 5 minutes whole cell lysate Lane3:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 10 minutes whole cell lysate Lane4:L02 treated with PBS (1xPBS, PH7.4) for 1 houre then treated with DMEM (10%FBS) for 15 minutes whole cell lysate