HDAC5 (phospho-S498) polyclonal antibody
Product Specifications
Background
HDAC5 is a member of the class II mammalian histone deacetylase family, which is structurally related to yeast HDA1. Human HDAC5 is composed of 1122 amino acid residues. The deacetylase domain of HDAC5 is located at the C-terminal half of the molecule. The N-terminal non-deacetylase domain does not show any significant homology with any published sequence.Both domains are required for HDAC5-mediated repression of gene transcription. HDAC5 interacts with a growing number of transcriptional factors including MEF2A as well as other HDAC proteins. The interacting complexes bind to specific regions of chromatin and regulate gene transcription in these regions.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Histone deacetylase 5; Short name=HD5; Antigen NY-CO-9; HDAC-5; KIAA0600
Swiss Prot
Q9UQL6
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human HDAC5 around the phosphorylation site of Serine 498.
Conjugation
Unconjugated
Related Pathways
Notch Signaling, Nuclear Receptor Signaling, Cell Cycle Control G1 S Checkpoint, Epigenetic Writers and Erasers of Histone H3, Epigenetic Writers and Erasers of Histones H2A H2B and H4
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylated
Molecular Weight
~ 140 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-HDAC5 (S498) polyclonal antibody detects endogenous levels of HDAC5 protein only when phosphorylated at Ser498.
Applications Notes
Western blot (WB) analysis of p-HDAC5 (S498) pAb at 1:1000 dilution Lane1:The Uterus tissue lysate of Rat (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug) Lane6:U-87MG whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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