Ezrin (phospho-Y353) polyclonal antibody

Product Specifications

Background

Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cytovillin; Villin-2; Villin2; p81; EZR; VIL2

Swiss Prot

P15311

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Ezrin around the phosphorylation site of Tyrosine 353.

Conjugation

Unconjugated

Related Pathways

Contribution of ECM and Cytoskeletal Factors to EMT, B cell receptor signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 90 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Ezrin (Y353) polyclonal antibody detects endogenous levels of Ezrin protein only when phosphorylated at Tyr353 .

Applications Notes

Western blot (WB) analysis of p-Ezrin (Y353) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PMVEC whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)